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picture1_Tube Method Of Blood Grouping Pdf 89798 | Bloodservices Pdf Abo Grouping


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File: Tube Method Of Blood Grouping Pdf 89798 | Bloodservices Pdf Abo Grouping
transfusion medicine quality manual standard operating procedure for abo grouping tube method standard operating procedure for abo grouping tube method provincial blood coordinating program 1 0 1 1 each recipient ...

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               Transfusion Medicine Quality Manual 
           Standard Operating Procedure for ABO Grouping 
                       Tube Method 
                            
           
           
           
           
           
           
           
           
           
           
           
           
         
         
         
         
         
         
         
         
         
         
         
         
         
         
                                                                  Standard Operating Procedure for 
                                                                       ABO Grouping Tube Method 
                                                                                                                                                                                
                               Provincial Blood  
                               Coordinating Program 
                                
                               1.0 	
	
	 
                                
                                           1.1  Each recipient blood sample for compatibility testing shall be tested for 
                                                    ABO group.  
                                                     
                                           1.2  The results of the red cell and plasma test must agree. Current and previous 
                                                    results should be compared to identify any ABO discrepancy. 
                                            
                                           1.3  All blood group discrepancy shall be resolved and the resolution 
                                                    documented before issuing red cells.  If transfusion is necessary before 
                                                    resolving the ABO discrepancy the recipient should receive group O red 
                                                    cells and AB plasma products. 
                                            
                                           1.4  Blood group testing for A, B and D antigens only is required for: 
                                                           1.4.1  Infants less than 4 months of age; and 
                                                           1.4.2  Confirmation of  the ABO group on donor units; 
                                                     
                               2.0 

 
                                
                                           Patient Identification and Specimen Labeling. Available at: 
                                           http://www.health.gov.nl.ca/health/bloodservices/pdf/patient_id_and_specimen_la
                                           beling.pdf 
                                
                                           Determining Specimen Suitability. Available at: 
                                           http://www.health.gov.nl.ca/health/bloodservices/pdf/determining_specimen_suit        
                                           ability.pdf 
                                
                                           Patient History Check. Available at: 
                                           http://www.health.gov.nl.ca/health/bloodservices/pdf/patient_history_check.pdf 
                                
                                           Preparation of Red Cell Suspensions. Available at: 
                                           http://www.health.gov.nl.ca/health/bloodservices/pdf/preparation_of_red_cell_sus
                                           pensions.pdf 
                                
                                           Quality Control of Reagents and Antisera. Available at: 
                                           http://www.health.gov.nl.ca/health/bloodservices/pdf/quality_control_of_reagents
                                           and_antisera_ver1.pdf 
                                
                                
                                _______________________________________________________________________ 
                                      This document may be incorporated into each Regional Policy/Procedure Manual. 
                                                                                                        
                                                                                                                                                      NL2010.-008 –TMQ 
                                                                                                                                                                Version: 3.0  
                                                                                                                                              Effective Date: 2015-04-03 
                                                                                                                                                                Page 2 of 11 
                              Standard Operating Procedure for 
                                 ABO Grouping Tube Method 
                                                                                 
              Provincial Blood  
              Coordinating Program 
               
                    Resolving ABO & Rh Blood Group Discrepancies. Available at: 
                    http://www.health.gov.nl.ca/health/bloodservices/pdf/resolving_abo_rh_blood_gr
                    oup_discrepancies.pdf 
               
              3.0  
               
                    3.1  All Transfusion Medicine Laboratory Technologists who are responsible for 
                        testing and reporting transfusion medicine samples.  
               
              4.0 



	
 
               
                    4.1  ABO blood groups are determined by phenotyping the recipient’s red cells 
                        for the presence or absence of A and B antigens on red cells and by testing 
                        the recipient’s plasma for the presence or absence of anti-A and anti-B. 
                         
                    4.2  ABO typing and ABO compatibility testing is the foundation of 
                        pretransfusion testing. 
                         
                    4.3  Transfusion of ABO incompatible blood can be associated with acute 
                        intravascular hemolysis, renal failure and death. 
                         
                    4.4  Routine tests to determine the ABO group consists of testing red cells with 
                        anti-A and anti-B (forward group) and testing the plasma with A  and B red 
                                                                       1
                        cells (reverse group). 
                         
                    4.5  Hemolysis is interpreted as a positive result. 
                         
                    4.6  Pre-washing the red cells is not necessary, however, if a discrepancy is          
                        detected the cells should be washed and the tests repeated. 
                         
                    4.7  Washed red cells may reduce false positive results associated with rouleaux 
                        or autoantibodies. 
                         
                    4.8  Positive reactions in the forward group characteristically demonstrate a 
                        grade three (3) to grade four (4) agglutination. Reactions in the reverse 
                        grouping (serum and reagent red cells) are often weaker. 
                         
                    4.9  Reactions in the forward group that demonstrate less than grade 2 reactions 
                        should be further investigated. 
                     
               _______________________________________________________________________ 
                  This document may be incorporated into each Regional Policy/Procedure Manual. 
                                                
                                                                    NL2010.-008 –TMQ 
                                                                         Version: 3.0  
                                                                 Effective Date: 2015-04-03 
                                                                         Page 3 of 11 
                                                                  Standard Operating Procedure for 
                                                                       ABO Grouping Tube Method 
                                                                                                                                                                                
                               Provincial Blood  
                               Coordinating Program 
                                                     
                                           4.10  The serum tests may be incubated at room temperature for 5 to 15 minutes 
                                                    to enhance weak reactions. 
                                                     
                                           4.11  A mixed field reaction maybe detected if a recipient has been transfused 
                                                    with ABO compatible red cells other than that of their own ABO group. 
                                                     
                                           4.12  False positive or negative results can be caused by variables such as: 
                                                       4.12.1  Improper technique 
                                                       4.12.2  Contaminated materials 
                                                       4.12.3  Omission of reagents or anti-sera 
                                                       4.12.4  Delays in testing 
                                                       4.12.5  Inadequate incubation time and temperature 
                                                       4.12.6  Inappropriate centrifugation 
                                                       4.12.7  Inappropriate or prolonged storage of red cells. 
                                                                    
                                           4.13  If performing the ABO grouping by automation follow manufacturer’s 
                                                    instructions for operation and resulting. 
                                
                               5.0  
                                
                                           5.1  Quality Control  
                                                    5.1.1  All reagents shall be used and controlled according to the 
                                                                manufacturer’s written instructions. 
                                                    5.1.2  All anti-sera must be visually inspected for contamination such as 
                                                                discoloration, cloudiness, turbidity and/or particulate matter. 
                                                    5.1.3  All reagent red cells must be visually inspected for hemolysis and/or 
                                                                discoloration. 
                                                    5.1.4  The results of the visual inspection, reagent lot number, expiry date, 
                                                                date of the inspection and the individual performing the inspection 
                                                                must be documented. 
                                                    5.1.5  The expiry date should be checked on each reagent used. Do not use 
                                                                reagents beyond expiry date. 
                                                     
                                _______________________________________________________________________ 
                                      This document may be incorporated into each Regional Policy/Procedure Manual. 
                                                                                                        
                                                                                                                                                      NL2010.-008 –TMQ 
                                                                                                                                                                Version: 3.0  
                                                                                                                                              Effective Date: 2015-04-03 
                                                                                                                                                                Page 4 of 11 
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