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picture1_Methods Of Isolation Of Bacteria Pdf 88060 | Isolation From Soil


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File: Methods Of Isolation Of Bacteria Pdf 88060 | Isolation From Soil
aim isolation of bacteria from soil by serial dilution method principle isolation is often essential for taxonomic and experimental work on micro organisms in the microbiological sense it is the ...

icon picture PDF Filetype PDF | Posted on 15 Sep 2022 | 3 years ago
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       Aim: Isolation of bacteria from soil by serial dilution method 
       Principle: Isolation is often essential for taxonomic and experimental work on micro-organisms. 
       In the microbiological sense, it is the process of separating a single species of microorganism 
       from its natural habitat and growing it by itself, without interference from other organisms, on a 
       sterile  substratum, i.e. in medium. The micro-organism can then be distinguished from other 
       species by its individual characters (even if artefacts of laboratory conditions) and propagated to 
       provide  experimental  material.  Methods  of  isolating  micro-organisms  from  a  natural 
       environment, such as soil, litter, air, or water, are numerous. 
       Serial dilution, as the name suggests, is a series of sequential dilutions that are performed to 
       convert a dense solution into a more usable concentration. In simple words, serial dilution is the 
       process of stepwise dilution of a solution with an associated dilution factor. In biology, serial 
       dilution is often associated with reducing the concentration of cells in a culture to simplify the 
       operation. 
        
       Material Required: 
       Nutrient agar media, petri dish, test tubes, pipette, incubator, autoclave, soil sample. 
        
       Method: 
         1.  Prepare 100 ml of nutrient agar media and autoclave it along with clean petri dishes.  
         2.  Meanwhile, arrange 7 test tubes in a row and add 9 ml distilled water to each tube. 
         3.  Mark the tubes as 10-1 upto 10-7 
         4.  Add 1 ml of sample in first test tube and mix well by vortexing. 
         5.  Now take 1 ml from first test tube and add it to the second test tube. Repeat the steps till 
          7th tube is reached. 
         6.  After autoclaving pour 15 - 18 ml of media in each petri dish inside the laminar air flow 
          and allow them to solidify. 
         7.  After the media is solidified, take 1 ml from any of the dilution and add it to the agar 
          plate.  
         8.  Incubate the plates at 37o C from 48 hours 
         9.  Observe the growth. 
       Result: 
        
       Precautions: 
        
                  
                        Always autoclave media. 
                        Never open autoclave media outside laminar air flow. 
                        Do not talk while working on laminar cabinet 
                  
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...Aim isolation of bacteria from soil by serial dilution method principle is often essential for taxonomic and experimental work on micro organisms in the microbiological sense it process separating a single species microorganism its natural habitat growing itself without interference other sterile substratum i e medium organism can then be distinguished individual characters even if artefacts laboratory conditions propagated to provide material methods isolating environment such as litter air or water are numerous name suggests series sequential dilutions that performed convert dense solution into more usable concentration simple words stepwise with an associated factor biology reducing cells culture simplify operation required nutrient agar media petri dish test tubes pipette incubator autoclave sample prepare ml along clean dishes meanwhile arrange row add distilled each tube mark upto first mix well vortexing now take second repeat steps till th reached after autoclaving pour inside ...

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