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Special Stains Handbook
Preface
Diapath S.p.A. is glad to introduce the “Special Stains Hand- ter” referring to the deparaffiniing steps in ylene or substi- o aoid undesirable specimen drying, we recommend to per- eagents for dehydration, hydration, clarification and mount-
book”. tutes and hydration ascending alcoholic scale with last step in form staining keeping the coerslip in an incubation bo. ing alcoholic scale, ylene or substitutes, mounting media,
In this handbook are collected the components and the main water of the section. In some cases the complete hydration is e suggest the use of control tests to erify that the protocol is coerslip
staining protocols of special stains kit. not necessary, but it will be mentioned, for eample, the step properly performed. lotting paper
Special stains are generally the non-routine stains applied to in which start the staining.” ehydrate, clear and mount with dditional glassware histological hoplin ar, glass stick,
histological sections or cytological preparations, able to show balsam” are the further steps in the ascending alcoholic scale he special stain kits are supplied in special packaging with rectangular ars for staining. e recommend the use of
specific tissue components, to differentiate cell types and de- and ylene or substitutes that allow a steady slide mounting. practical containers which allow an application drop by drop on clean glassware. In case of protocols inoling the use of re-
tect the presence of any microorganisms. If it is necessary the use of a water-based mounting media, it the specimen, ensuring a considerable material saing. agents containing siler, don’t use metallic obects.
Special stains are, therefore, a good tool to support the re- will be specified in the staining protocol. The Technical Data Sheet with the staining protocol is at-
search techniues normally used to define the diagnostic pa- he suggested times are an approimation and could change tached to each package.
tient profile. according to specific needs.
Here follows the necessary equipment to be used during the e hope that this handbook is a aluable guide for your his-
his handbook proides practical adice for getting the most he special stains kits are planned for about tests, staining protocols (not supplied in the kit): tological procedures and a aluable help to perform special
from different staining techniues and offers the best solu- considering that to coer a specimen are necessary about staining.
tions to oercome the most common mistakes. drops. lease note that the drop number changes according to Incubation bo with rack for horiontal staining. lternatie-
specimen sie. ly, we suggest the use of an humidified slide bo on the bot-
efore proceeding, we point out some important notes for a
n reuest, are aailable kits with larger packaging to perform tom with some blotting paper soaked in water
uick reading. staining in immersion. Sueee bottle with distilled water or ertical hoplin ar filled
In each staining protocols and in the corresponding data sheet Some solutions should be prepared only shortly before their with ml of distilled water for the washing steps
is used the wording “ eparaffinie and hydrate to distilled wa- use to aoid possible degrade that could affect staining result. eagents for deparaffiniing ylene or substitutes ood eading
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RECEIVE IN REAL TIME ALL DIApATh SpECIAL STAINS UpDATINGS
Diapath Special Stains Handbook is a constantly updating catalogue. Visit our website www.diapath.com,
register in the Reserved Area and ask to be a member of our mailing list to receive in real time all Diapath
special stains updatings.
ontact our roduct Specialists at specialstaisdiapath.com to receive support, information and eplanations
concerning Diapath special stains protocols and methods.
he image shades reported here can change according to the employed printing process.
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