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picture1_Southern Blotting Slideshare 67001 | Biologyformajorsi 14 Modernbiology


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File: Southern Blotting Slideshare 67001 | Biologyformajorsi 14 Modernbiology
dna extraction gel electrophoresis because nucleic acids are negatively charged ions at neutral or basic ph in an aqueous environment they can be mobilized by an electric field gel electrophoresis ...

icon picture PPTX Filetype Power Point PPTX | Posted on 28 Aug 2022 | 3 years ago
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   DNA Extraction
          Gel Electrophoresis
           •  Because nucleic acids are 
              negatively charged ions at 
              neutral or basic pH in an 
              aqueous environment, they 
              can be mobilized by an 
              electric field. Gel 
              electrophoresis is a 
              technique used to separate 
              molecules on the basis of 
              size, using this charge. 
          PCR
           •  PCR is used to 
              amplify a specific 
              sequence of 
              DNA.
     Reverse Transcriptase PCR
     DNA fragments can also be amplified from an RNA template in a process 
     called reverse transcriptase PCR (RT-PCR). The first step is to recreate 
     the original DNA template strand (called cDNA) by applying DNA nucleotides 
     to the mRNA in a process called reverse transcription. This requires the 
     presence of an enzyme called reverse transcriptase. After the cDNA is made, 
     regular PCR can be used to amplify it.
   Southern Blotting
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...Dna extraction gel electrophoresis because nucleic acids are negatively charged ions at neutral or basic ph in an aqueous environment they can be mobilized by electric field is a technique used to separate molecules on the basis of size using this charge pcr amplify specific sequence reverse transcriptase fragments also amplified from rna template process called rt first step recreate original strand cdna applying nucleotides mrna transcription requires presence enzyme after made regular it southern blotting...

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